Trypsin Inhibitor Antikörper (Biotin)

Produktnummer ABIN1607819

Hersteller Produkt- Nr.: 200-4687-0100

Dieses Anti-Trypsin Inhibitor-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Trypsin Inhibitor in WB, ELISA und DB. Geeignet für Lima bean.

Kurzübersicht für Trypsin Inhibitor Antikörper (Biotin) (ABIN1607819)

Target: Trypsin Inhibitor

Reaktivität: Lima bean

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Trypsin Inhibitor Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA, Dot Blot (DB)

Produktdetails anti-Trypsin Inhibitor Antikörper (Biotin)

Hersteller Produkt- Nr.: 200-4687-0100

Hersteller: Rockland

Verwendungszweck: Trypsin Inhibitor Antibody Biotin Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Trypsin Inhibitor [Lima Bean].

Produktmerkmale: Synonyms: rabbit anti-trypsin inhibitor antibody biotin conjugation, biotin conjugated rabbit anti-trypsin inhibitor antibody, 25 kDa trypsin inhibitor (Protease inhibitor 15 preproprotein) antibody, CRISP8 antibody, KTi antibody, Kti S antibody, KTi3 antibody, KtiS antibody, Kunitz trypsin inhibitor antibody, Kunitz type trypsin inhibitor A antibody

Aufreinigung: Anti-Trypsin Inhibitor antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Trypsin Inhibitor [Lima Bean]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Trypsin Inhibitor antibody has been tested by dot blot and assayed against 1.0 μg of Trypsin Inhibitor in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:6,000 to 1:26,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:250

ELISA Dilution: 1:500 - 1:2,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 10 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Trypsin Inhibitor

Target: Trypsin Inhibitor

Hintergrund: Background: Soy Bean Trypsin Inhibitor is a serine protease inhibitor, an inhibitor of trypsin, therefore preventing the further breakdown of proteins.

Gen-ID: 547831

UniProt: P01070